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A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses
Joint Authors
Lecollinet, Sylvie
Beck, Cécile
Desprès, Philippe
Paulous, Sylvie
Vanhomwegen, Jessica
Lowenski, Steeve
Nowotny, Norbert
Durand, Benoit
Garnier, Annabelle
Blaise-Boisseau, Sandra
Guitton, Edouard
Yamanaka, Takashi
Zientara, Stéphan
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-17
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
West Nile virus (WNV), Japanese encephalitis virus (JEV), and tick-borne encephalitis virus (TBEV) are flaviviruses responsible for severe neuroinvasive infections in humans and horses.
The confirmation of flavivirus infections is mostly based on rapid serological tests such as enzyme-linked immunosorbent assays (ELISAs).
These tests suffer from poor specificity, mainly due to antigenic cross-reactivity among flavivirus members.
Robust diagnosis therefore needs to be validated through virus neutralisation tests (VNTs) which are time-consuming and require BSL3 facilities.
The flavivirus envelope (E) glycoprotein ectodomain is composed of three domains (D) named DI, DII, and DIII, with EDIII containing virus-specific epitopes.
In order to improve the serological differentiation of flavivirus infections, the recombinant soluble ectodomain of WNV E (WNV.sE) and EDIIIs (rEDIIIs) of WNV, JEV, and TBEV were synthesised using the Drosophila S2 expression system.
Purified antigens were covalently bonded to fluorescent beads.
The microspheres coupled to WNV.sE or rEDIIIs were assayed with about 300 equine immune sera from natural and experimental flavivirus infections and 172 nonimmune equine sera as negative controls.
rEDIII-coupled microspheres captured specific antibodies against WNV, TBEV, or JEV in positive horse sera.
This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases.
American Psychological Association (APA)
Beck, Cécile& Desprès, Philippe& Paulous, Sylvie& Vanhomwegen, Jessica& Lowenski, Steeve& Nowotny, Norbert…[et al.]. 2015. A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses. BioMed Research International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1056353
Modern Language Association (MLA)
Beck, Cécile…[et al.]. A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses. BioMed Research International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1056353
American Medical Association (AMA)
Beck, Cécile& Desprès, Philippe& Paulous, Sylvie& Vanhomwegen, Jessica& Lowenski, Steeve& Nowotny, Norbert…[et al.]. A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1056353
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056353