Evaluation of Transduction Properties of an Adenovirus Vector in Neonatal Mice
المؤلفون المشاركون
Sakurai, Fuminori
Iizuka, Shunsuke
Shimizu, Kahori
Ohashi, Kazuo
Nakamura, Shin-ichiro
Tachibana, Masashi
Mizuguchi, Hiroyuki
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-13
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
In gene therapy for congenital disorders, treatments during neonate and infant stages are promising.
Replication-incompetent adenovirus (Ad) vectors have been used in gene therapy studies of genetic disorders; however, the transduction properties of Ad vectors in neonates and infants have not been fully examined.
Accordingly, this study examined the properties of Ad vector-mediated transduction in neonatal mice.
A first-generation Ad vector containing a cytomegalovirus (CMV) promoter-driven luciferase expression cassette was administered to neonatal mice on the second day of life via retro-orbital sinus.
The highest Ad vector genome copy numbers and transgene expression were found in the neonatal liver.
The neonatal heart exhibited the second highest levels of transgene expression among the organs examined.
There was an approximately 1500-fold difference in the transgene expression levels between the adult liver and heart, while the neonatal liver exhibited only an approximately 30-fold higher level of transgene expression than the neonatal heart.
A liver-specific promoter for firefly luciferase expression conferred a more than 100-fold higher luciferase expression in the liver relative to the other organs.
No apparent hepatotoxicity was observed in neonatal mice following Ad vector administration.
These findings should provide valuable information for gene therapy using Ad vectors in neonates and infants.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Iizuka, Shunsuke& Sakurai, Fuminori& Shimizu, Kahori& Ohashi, Kazuo& Nakamura, Shin-ichiro& Tachibana, Masashi…[et al.]. 2015. Evaluation of Transduction Properties of an Adenovirus Vector in Neonatal Mice. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056384
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Iizuka, Shunsuke…[et al.]. Evaluation of Transduction Properties of an Adenovirus Vector in Neonatal Mice. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056384
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Iizuka, Shunsuke& Sakurai, Fuminori& Shimizu, Kahori& Ohashi, Kazuo& Nakamura, Shin-ichiro& Tachibana, Masashi…[et al.]. Evaluation of Transduction Properties of an Adenovirus Vector in Neonatal Mice. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056384
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056384
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر