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Evaluation of Transduction Properties of an Adenovirus Vector in Neonatal Mice
Joint Authors
Sakurai, Fuminori
Iizuka, Shunsuke
Shimizu, Kahori
Ohashi, Kazuo
Nakamura, Shin-ichiro
Tachibana, Masashi
Mizuguchi, Hiroyuki
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In gene therapy for congenital disorders, treatments during neonate and infant stages are promising.
Replication-incompetent adenovirus (Ad) vectors have been used in gene therapy studies of genetic disorders; however, the transduction properties of Ad vectors in neonates and infants have not been fully examined.
Accordingly, this study examined the properties of Ad vector-mediated transduction in neonatal mice.
A first-generation Ad vector containing a cytomegalovirus (CMV) promoter-driven luciferase expression cassette was administered to neonatal mice on the second day of life via retro-orbital sinus.
The highest Ad vector genome copy numbers and transgene expression were found in the neonatal liver.
The neonatal heart exhibited the second highest levels of transgene expression among the organs examined.
There was an approximately 1500-fold difference in the transgene expression levels between the adult liver and heart, while the neonatal liver exhibited only an approximately 30-fold higher level of transgene expression than the neonatal heart.
A liver-specific promoter for firefly luciferase expression conferred a more than 100-fold higher luciferase expression in the liver relative to the other organs.
No apparent hepatotoxicity was observed in neonatal mice following Ad vector administration.
These findings should provide valuable information for gene therapy using Ad vectors in neonates and infants.
American Psychological Association (APA)
Iizuka, Shunsuke& Sakurai, Fuminori& Shimizu, Kahori& Ohashi, Kazuo& Nakamura, Shin-ichiro& Tachibana, Masashi…[et al.]. 2015. Evaluation of Transduction Properties of an Adenovirus Vector in Neonatal Mice. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056384
Modern Language Association (MLA)
Iizuka, Shunsuke…[et al.]. Evaluation of Transduction Properties of an Adenovirus Vector in Neonatal Mice. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1056384
American Medical Association (AMA)
Iizuka, Shunsuke& Sakurai, Fuminori& Shimizu, Kahori& Ohashi, Kazuo& Nakamura, Shin-ichiro& Tachibana, Masashi…[et al.]. Evaluation of Transduction Properties of an Adenovirus Vector in Neonatal Mice. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1056384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056384