Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867)‎ (Acari: Tetranychidae)‎, and Its Response to β-Sitosterol

المؤلفون المشاركون

Bu, Chunya
Li, Jinling
Wang, Xiao-Qin
Shi, Guanglu
Peng, Bo
Han, Jingyu
Gao, Pin
Wang, Younian

المصدر

BioMed Research International

العدد

المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2015-05-11

دولة النشر

مصر

عدد الصفحات

12

التخصصات الرئيسية

الطب البشري

الملخص EN

Tetranychus cinnabarinus (Acari: Tetranychidae) is a worldwide polyphagous agricultural pest that has the title of resistance champion among arthropods.

We reported previously the identification of the acaricidal compound β-sitosterol from Mentha piperita and Inula japonica.

However, the acaricidal mechanism of β-sitosterol is unclear.

Due to the limited genetic research carried out, we de novo assembled the transcriptome of T.

cinnabarinus using Illumina sequencing and conducted a differential expression analysis of control and β-sitosterol-treated mites.

In total, we obtained >5.4 G high-quality bases for each sample with unprecedented sequencing depth and assembled them into 22,941 unigenes.

We identified 617 xenobiotic metabolism-related genes involved in detoxification, binding, and transporting of xenobiotics.

A highly expanded xenobiotic metabolic system was found in mites.

T.

cinnabarinus detoxification genes—including carboxyl/cholinesterase and ABC transporter class C—were upregulated after β-sitosterol treatment.

Defense-related proteins, such as Toll-like receptor, legumain, and serine proteases, were also activated.

Furthermore, other important genes—such as the chloride channel protein, cytochrome b, carboxypeptidase, peritrophic membrane chitin binding protein, and calphostin—may also play important roles in mites’ response to β-sitosterol.

Our results demonstrate that high-throughput-omics tool facilitates identification of xenobiotic metabolism-related genes and illustration of the acaricidal mechanisms of β-sitosterol.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Bu, Chunya& Li, Jinling& Wang, Xiao-Qin& Shi, Guanglu& Peng, Bo& Han, Jingyu…[et al.]. 2015. Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae), and Its Response to β-Sitosterol. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1056752

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Bu, Chunya…[et al.]. Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae), and Its Response to β-Sitosterol. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1056752

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Bu, Chunya& Li, Jinling& Wang, Xiao-Qin& Shi, Guanglu& Peng, Bo& Han, Jingyu…[et al.]. Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae), and Its Response to β-Sitosterol. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1056752

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1056752