Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae), and Its Response to β-Sitosterol
Joint Authors
Bu, Chunya
Li, Jinling
Wang, Xiao-Qin
Shi, Guanglu
Peng, Bo
Han, Jingyu
Gao, Pin
Wang, Younian
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Tetranychus cinnabarinus (Acari: Tetranychidae) is a worldwide polyphagous agricultural pest that has the title of resistance champion among arthropods.
We reported previously the identification of the acaricidal compound β-sitosterol from Mentha piperita and Inula japonica.
However, the acaricidal mechanism of β-sitosterol is unclear.
Due to the limited genetic research carried out, we de novo assembled the transcriptome of T.
cinnabarinus using Illumina sequencing and conducted a differential expression analysis of control and β-sitosterol-treated mites.
In total, we obtained >5.4 G high-quality bases for each sample with unprecedented sequencing depth and assembled them into 22,941 unigenes.
We identified 617 xenobiotic metabolism-related genes involved in detoxification, binding, and transporting of xenobiotics.
A highly expanded xenobiotic metabolic system was found in mites.
T.
cinnabarinus detoxification genes—including carboxyl/cholinesterase and ABC transporter class C—were upregulated after β-sitosterol treatment.
Defense-related proteins, such as Toll-like receptor, legumain, and serine proteases, were also activated.
Furthermore, other important genes—such as the chloride channel protein, cytochrome b, carboxypeptidase, peritrophic membrane chitin binding protein, and calphostin—may also play important roles in mites’ response to β-sitosterol.
Our results demonstrate that high-throughput-omics tool facilitates identification of xenobiotic metabolism-related genes and illustration of the acaricidal mechanisms of β-sitosterol.
American Psychological Association (APA)
Bu, Chunya& Li, Jinling& Wang, Xiao-Qin& Shi, Guanglu& Peng, Bo& Han, Jingyu…[et al.]. 2015. Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae), and Its Response to β-Sitosterol. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1056752
Modern Language Association (MLA)
Bu, Chunya…[et al.]. Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae), and Its Response to β-Sitosterol. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1056752
American Medical Association (AMA)
Bu, Chunya& Li, Jinling& Wang, Xiao-Qin& Shi, Guanglu& Peng, Bo& Han, Jingyu…[et al.]. Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae), and Its Response to β-Sitosterol. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1056752
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056752