Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent Effect
المؤلفون المشاركون
Montes-Fonseca, Silvia Lorena
Luna-Velasco, Antonia
González Horta, Carmen
Sánchez-Ramírez, Blanca
Arzate-Quintana, Carlos
Silva-Cazares, Macrina Beatriz
Orrantia-Borunda, E.
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-17
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Carbon nanotubes (CNTs) are used as carriers in medicine due to their ability to be functionalized with chemical substances.
However, cytotoxicity analysis is required prior to use for in vivo models.
The aim of this study was to evaluate the cytotoxic effect of CNTs functionalized with a 46 kDa surface protein from Entamoeba histolytica (P46-CNTs) on J774A macrophages.
With this purpose, CNTs were synthesized by spray pyrolysis and purified (P-CNTs) using sonication for 48 h.
A 46 kDa protein, with a 4.6–5.4 pI range, was isolated from E.
histolytica HM1:IMSS strain trophozoites using an OFFGEL system.
The P-CNTs were functionalized with the purified 46 kDa protein, classified according to their degree of functionalization, and characterized by Raman and Infrared spectroscopy.
In vitro cytotoxicity was evaluated by MTT, apoptosis, and morphological assays.
The results demonstrated that P46-CNTs exhibited cytotoxicity dependent upon the functionalized grade.
Contrary to what was expected, P46-CNTs with a high grade of functionalization were more toxic to J774 macrophages than P46-CNTs with a low grade of functionalization, than P-CNTs, and had a similar level of toxicity as UP-CNT.
This suggests that the nature of the functionalized protein plays a key role in the cytotoxicity of these nanoparticles.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Montes-Fonseca, Silvia Lorena& Sánchez-Ramírez, Blanca& Luna-Velasco, Antonia& Arzate-Quintana, Carlos& Silva-Cazares, Macrina Beatriz& González Horta, Carmen…[et al.]. 2015. Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent Effect. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056760
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Montes-Fonseca, Silvia Lorena…[et al.]. Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent Effect. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1056760
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Montes-Fonseca, Silvia Lorena& Sánchez-Ramírez, Blanca& Luna-Velasco, Antonia& Arzate-Quintana, Carlos& Silva-Cazares, Macrina Beatriz& González Horta, Carmen…[et al.]. Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent Effect. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056760
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056760
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر