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Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent Effect
Joint Authors
Montes-Fonseca, Silvia Lorena
Luna-Velasco, Antonia
González Horta, Carmen
Sánchez-Ramírez, Blanca
Arzate-Quintana, Carlos
Silva-Cazares, Macrina Beatriz
Orrantia-Borunda, E.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Carbon nanotubes (CNTs) are used as carriers in medicine due to their ability to be functionalized with chemical substances.
However, cytotoxicity analysis is required prior to use for in vivo models.
The aim of this study was to evaluate the cytotoxic effect of CNTs functionalized with a 46 kDa surface protein from Entamoeba histolytica (P46-CNTs) on J774A macrophages.
With this purpose, CNTs were synthesized by spray pyrolysis and purified (P-CNTs) using sonication for 48 h.
A 46 kDa protein, with a 4.6–5.4 pI range, was isolated from E.
histolytica HM1:IMSS strain trophozoites using an OFFGEL system.
The P-CNTs were functionalized with the purified 46 kDa protein, classified according to their degree of functionalization, and characterized by Raman and Infrared spectroscopy.
In vitro cytotoxicity was evaluated by MTT, apoptosis, and morphological assays.
The results demonstrated that P46-CNTs exhibited cytotoxicity dependent upon the functionalized grade.
Contrary to what was expected, P46-CNTs with a high grade of functionalization were more toxic to J774 macrophages than P46-CNTs with a low grade of functionalization, than P-CNTs, and had a similar level of toxicity as UP-CNT.
This suggests that the nature of the functionalized protein plays a key role in the cytotoxicity of these nanoparticles.
American Psychological Association (APA)
Montes-Fonseca, Silvia Lorena& Sánchez-Ramírez, Blanca& Luna-Velasco, Antonia& Arzate-Quintana, Carlos& Silva-Cazares, Macrina Beatriz& González Horta, Carmen…[et al.]. 2015. Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent Effect. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056760
Modern Language Association (MLA)
Montes-Fonseca, Silvia Lorena…[et al.]. Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent Effect. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1056760
American Medical Association (AMA)
Montes-Fonseca, Silvia Lorena& Sánchez-Ramírez, Blanca& Luna-Velasco, Antonia& Arzate-Quintana, Carlos& Silva-Cazares, Macrina Beatriz& González Horta, Carmen…[et al.]. Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent Effect. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1056760
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056760