POD-1TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells
المؤلفون المشاركون
Ferraz-de-Souza, Bruno
França, Monica Malheiros
Lerario, Antonio Marcondes
Lotfi, Claudimara Ferini Pacicco
Fragoso, Maria Candida Barisson Villares
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-09-02
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
POD-1/TCF21 may play a crucial role in adrenal and gonadal homeostasis and represses Sf-1/SF-1 expression in adrenocortical tumor cells.
SF-1 and LRH-1 are members of the Fzt-F1 subfamily of nuclear receptors.
LRH-1 is involved in several biological processes, and both LRH-1 and its repressor SHP are involved in many types of cancer.
In order to assess whether POD-1 can regulate LRH-1 via the same mechanism that regulates SF-1, we analyzed the endogenous mRNA levels of POD-1, SHP, and LRH-1 in hepatocarcinoma and adrenocortical tumor cells using qRT-PCR.
Hereafter, these tumor cells were transiently transfected with pCMVMycPod-1, and the effect of POD-1 overexpression on E-box elements in the LRH-1 and SHP promoter region were analyzed by ChIP assay.
Also, Cyclin E1 protein expression was analyzed to detect cell cycle progression.
We found that POD-1 overexpression significantly decreased SHP/SHP mRNA and protein levels through POD-1 binding to the E-box sequence in the SHP promoter.
Decreased SHP expression affected LRH-1 regulation and increased Cyclin E1.
These findings show that POD-1/TCF21 regulates SF-1 and LRH-1 by distinct mechanisms, contributing to the understanding of POD-1 involvement and its mechanisms of action in adrenal and liver tumorigenesis, which could lead to the discovery of relevant biomarkers.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
França, Monica Malheiros& Ferraz-de-Souza, Bruno& Lerario, Antonio Marcondes& Fragoso, Maria Candida Barisson Villares& Lotfi, Claudimara Ferini Pacicco. 2015. POD-1TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056956
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
França, Monica Malheiros…[et al.]. POD-1TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056956
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
França, Monica Malheiros& Ferraz-de-Souza, Bruno& Lerario, Antonio Marcondes& Fragoso, Maria Candida Barisson Villares& Lotfi, Claudimara Ferini Pacicco. POD-1TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056956
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056956
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر