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POD-1TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells
Joint Authors
Ferraz-de-Souza, Bruno
França, Monica Malheiros
Lerario, Antonio Marcondes
Lotfi, Claudimara Ferini Pacicco
Fragoso, Maria Candida Barisson Villares
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
POD-1/TCF21 may play a crucial role in adrenal and gonadal homeostasis and represses Sf-1/SF-1 expression in adrenocortical tumor cells.
SF-1 and LRH-1 are members of the Fzt-F1 subfamily of nuclear receptors.
LRH-1 is involved in several biological processes, and both LRH-1 and its repressor SHP are involved in many types of cancer.
In order to assess whether POD-1 can regulate LRH-1 via the same mechanism that regulates SF-1, we analyzed the endogenous mRNA levels of POD-1, SHP, and LRH-1 in hepatocarcinoma and adrenocortical tumor cells using qRT-PCR.
Hereafter, these tumor cells were transiently transfected with pCMVMycPod-1, and the effect of POD-1 overexpression on E-box elements in the LRH-1 and SHP promoter region were analyzed by ChIP assay.
Also, Cyclin E1 protein expression was analyzed to detect cell cycle progression.
We found that POD-1 overexpression significantly decreased SHP/SHP mRNA and protein levels through POD-1 binding to the E-box sequence in the SHP promoter.
Decreased SHP expression affected LRH-1 regulation and increased Cyclin E1.
These findings show that POD-1/TCF21 regulates SF-1 and LRH-1 by distinct mechanisms, contributing to the understanding of POD-1 involvement and its mechanisms of action in adrenal and liver tumorigenesis, which could lead to the discovery of relevant biomarkers.
American Psychological Association (APA)
França, Monica Malheiros& Ferraz-de-Souza, Bruno& Lerario, Antonio Marcondes& Fragoso, Maria Candida Barisson Villares& Lotfi, Claudimara Ferini Pacicco. 2015. POD-1TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056956
Modern Language Association (MLA)
França, Monica Malheiros…[et al.]. POD-1TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1056956
American Medical Association (AMA)
França, Monica Malheiros& Ferraz-de-Souza, Bruno& Lerario, Antonio Marcondes& Fragoso, Maria Candida Barisson Villares& Lotfi, Claudimara Ferini Pacicco. POD-1TCF21 Reduces SHP Expression, Affecting LRH-1 Regulation and Cell Cycle Balance in Adrenocortical and Hepatocarcinoma Tumor Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1056956
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1056956