Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods
المؤلفون المشاركون
Baumert, Joseph L.
Taylor, Steve L.
Koppelman, Stef J.
Söylemez, Gülsen
Niemann, Lynn
Gaskin, Ferdelie E.
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-09
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Small amounts of sesame can trigger allergic reactions in sesame-allergic patients.
Because sesame is a widely used food ingredient, analytical methods are needed to support quality control and food safety programs in the food industry.
In this study, polyclonal antibodies against sesame seed proteins were raised, and an enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantification of sesame seed residue in food.
A comparison was made between this ELISA and other assays, particularly focusing on recovery of sesame seed residue from different food matrices.
The developed ELISA is sensitive with a lower limit of quantification of 0.5 ppm and shows essentially no cross-reactivity with other foods or food ingredients (92 tested).
The ELISA has a good recovery for analyzing sesame-based tahini in peanut butter, outperforming one other test.
In a baked bread matrix, the ELISA has a low recovery, while two other assays perform better.
We conclude that a sensitive and specific ELISA can be constructed based on polyclonal antibodies, which is suitable for detection of small amounts of sesame seed relevant for highly allergic patients.
Furthermore, we conclude that different food products may require different assays to ensure adequate quantification of sesame.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Koppelman, Stef J.& Söylemez, Gülsen& Niemann, Lynn& Gaskin, Ferdelie E.& Baumert, Joseph L.& Taylor, Steve L.. 2015. Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057013
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Koppelman, Stef J.…[et al.]. Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1057013
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Koppelman, Stef J.& Söylemez, Gülsen& Niemann, Lynn& Gaskin, Ferdelie E.& Baumert, Joseph L.& Taylor, Steve L.. Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057013
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1057013
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر