Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods
Joint Authors
Baumert, Joseph L.
Taylor, Steve L.
Koppelman, Stef J.
Söylemez, Gülsen
Niemann, Lynn
Gaskin, Ferdelie E.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Small amounts of sesame can trigger allergic reactions in sesame-allergic patients.
Because sesame is a widely used food ingredient, analytical methods are needed to support quality control and food safety programs in the food industry.
In this study, polyclonal antibodies against sesame seed proteins were raised, and an enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantification of sesame seed residue in food.
A comparison was made between this ELISA and other assays, particularly focusing on recovery of sesame seed residue from different food matrices.
The developed ELISA is sensitive with a lower limit of quantification of 0.5 ppm and shows essentially no cross-reactivity with other foods or food ingredients (92 tested).
The ELISA has a good recovery for analyzing sesame-based tahini in peanut butter, outperforming one other test.
In a baked bread matrix, the ELISA has a low recovery, while two other assays perform better.
We conclude that a sensitive and specific ELISA can be constructed based on polyclonal antibodies, which is suitable for detection of small amounts of sesame seed relevant for highly allergic patients.
Furthermore, we conclude that different food products may require different assays to ensure adequate quantification of sesame.
American Psychological Association (APA)
Koppelman, Stef J.& Söylemez, Gülsen& Niemann, Lynn& Gaskin, Ferdelie E.& Baumert, Joseph L.& Taylor, Steve L.. 2015. Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057013
Modern Language Association (MLA)
Koppelman, Stef J.…[et al.]. Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1057013
American Medical Association (AMA)
Koppelman, Stef J.& Söylemez, Gülsen& Niemann, Lynn& Gaskin, Ferdelie E.& Baumert, Joseph L.& Taylor, Steve L.. Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Sesame Seed in Foods. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1057013
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057013