Endogenous Two-Photon Excited Fluorescence Provides Label-Free Visualization of the Inflammatory Response in the Rodent Spinal Cord
المؤلفون المشاركون
Kirsch, Matthias
Neuwirth, Ales
Uckermann, Ortrud
Galli, Roberta
Beiermeister, Rudolf
Sitoci-Ficici, Kerim-Hakan
Later, Robert
Leipnitz, Elke
Koch, Edmund
Chavakis, Triantafyllos
Schackert, Gabriele
Steiner, Gerald
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-08-18
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Activation of CNS resident microglia and invasion of external macrophages plays a central role in spinal cord injuries and diseases.
Multiphoton microscopy based on intrinsic tissue properties offers the possibility of label-free imaging and has the potential to be applied in vivo.
In this work, we analyzed cellular structures displaying endogenous two-photon excited fluorescence (TPEF) in the pathologic spinal cord.
It was compared qualitatively and quantitatively to Iba1 and CD68 immunohistochemical staining in two models: rat spinal cord injury and mouse encephalomyelitis.
The extent of tissue damage was retrieved by coherent anti-Stokes Raman scattering (CARS) and second harmonic generation imaging.
The pattern of CD68-positive cells representing postinjury activated microglia/macrophages was colocalized to the TPEF signal.
Iba1-positive microglia were found in areas lacking any TPEF signal.
In peripheral areas of inflammation, we found similar numbers of CD68-positive microglia/macrophages and TPEF-positive structures while the number of Iba1-positive cells was significantly higher.
Therefore, we conclude that multiphoton imaging of unstained spinal cord tissue enables retrieving the extent of microglia activation by acquisition of endogenous TPEF.
Future application of this technique in vivo will enable monitoring inflammatory responses of the nervous system allowing new insights into degenerative and regenerative processes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Uckermann, Ortrud& Galli, Roberta& Beiermeister, Rudolf& Sitoci-Ficici, Kerim-Hakan& Later, Robert& Leipnitz, Elke…[et al.]. 2015. Endogenous Two-Photon Excited Fluorescence Provides Label-Free Visualization of the Inflammatory Response in the Rodent Spinal Cord. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057038
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Uckermann, Ortrud…[et al.]. Endogenous Two-Photon Excited Fluorescence Provides Label-Free Visualization of the Inflammatory Response in the Rodent Spinal Cord. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1057038
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Uckermann, Ortrud& Galli, Roberta& Beiermeister, Rudolf& Sitoci-Ficici, Kerim-Hakan& Later, Robert& Leipnitz, Elke…[et al.]. Endogenous Two-Photon Excited Fluorescence Provides Label-Free Visualization of the Inflammatory Response in the Rodent Spinal Cord. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057038
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1057038
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر