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Endogenous Two-Photon Excited Fluorescence Provides Label-Free Visualization of the Inflammatory Response in the Rodent Spinal Cord
Joint Authors
Kirsch, Matthias
Neuwirth, Ales
Uckermann, Ortrud
Galli, Roberta
Beiermeister, Rudolf
Sitoci-Ficici, Kerim-Hakan
Later, Robert
Leipnitz, Elke
Koch, Edmund
Chavakis, Triantafyllos
Schackert, Gabriele
Steiner, Gerald
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Activation of CNS resident microglia and invasion of external macrophages plays a central role in spinal cord injuries and diseases.
Multiphoton microscopy based on intrinsic tissue properties offers the possibility of label-free imaging and has the potential to be applied in vivo.
In this work, we analyzed cellular structures displaying endogenous two-photon excited fluorescence (TPEF) in the pathologic spinal cord.
It was compared qualitatively and quantitatively to Iba1 and CD68 immunohistochemical staining in two models: rat spinal cord injury and mouse encephalomyelitis.
The extent of tissue damage was retrieved by coherent anti-Stokes Raman scattering (CARS) and second harmonic generation imaging.
The pattern of CD68-positive cells representing postinjury activated microglia/macrophages was colocalized to the TPEF signal.
Iba1-positive microglia were found in areas lacking any TPEF signal.
In peripheral areas of inflammation, we found similar numbers of CD68-positive microglia/macrophages and TPEF-positive structures while the number of Iba1-positive cells was significantly higher.
Therefore, we conclude that multiphoton imaging of unstained spinal cord tissue enables retrieving the extent of microglia activation by acquisition of endogenous TPEF.
Future application of this technique in vivo will enable monitoring inflammatory responses of the nervous system allowing new insights into degenerative and regenerative processes.
American Psychological Association (APA)
Uckermann, Ortrud& Galli, Roberta& Beiermeister, Rudolf& Sitoci-Ficici, Kerim-Hakan& Later, Robert& Leipnitz, Elke…[et al.]. 2015. Endogenous Two-Photon Excited Fluorescence Provides Label-Free Visualization of the Inflammatory Response in the Rodent Spinal Cord. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057038
Modern Language Association (MLA)
Uckermann, Ortrud…[et al.]. Endogenous Two-Photon Excited Fluorescence Provides Label-Free Visualization of the Inflammatory Response in the Rodent Spinal Cord. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1057038
American Medical Association (AMA)
Uckermann, Ortrud& Galli, Roberta& Beiermeister, Rudolf& Sitoci-Ficici, Kerim-Hakan& Later, Robert& Leipnitz, Elke…[et al.]. Endogenous Two-Photon Excited Fluorescence Provides Label-Free Visualization of the Inflammatory Response in the Rodent Spinal Cord. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1057038
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057038