Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design
المؤلفون المشاركون
Hutson, Christina L.
Carroll, Darin S.
Gallardo-Romero, Nadia
Drew, Clifton
Nagy, Tamas
Hughes, Christine
Olson, Victoria A.
Sanders, Jeanine
Patel, Nishi
Smith, Scott K.
Karem, Kevin
Damon, Inger K.
Zaki, Sherif R.
Keckler, M. Shannon
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-19، 19ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-08-24
دولة النشر
مصر
عدد الصفحات
19
التخصصات الرئيسية
الملخص EN
Monkeypox virus (MPXV) infection of the prairie dog is valuable to studying systemic orthopoxvirus disease.
To further characterize differences in MPXV clade pathogenesis, groups of prairie dogs were intranasally infected (8×103 p.f.u.) with Congo Basin (CB) or West African (WA) MPXV, and 28 tissues were harvested on days 2, 4, 6, 9, 12, 17, and 24 postinfection.
Samples were evaluated for the presence of virus and gross and microscopic lesions.
Virus was recovered from nasal mucosa, oropharyngeal lymph nodes, and spleen earlier in CB challenged animals (day 4) than WA challenged animals (day 6).
For both groups, primary viremia (indicated by viral DNA) was seen on days 6–9 through day 17.
CB MPXV spread more rapidly, accumulated to greater levels, and caused greater morbidity in animals compared to WA MPXV.
Histopathology and immunohistochemistry (IHC) findings, however, were similar.
Two animals that succumbed to disease demonstrated abundant viral antigen in all organs tested, except for brain.
Dual-IHC staining of select liver and spleen sections showed that apoptotic cells (identified by TUNEL) tended to colocalize with poxvirus antigen.
Interestingly splenocytes were labelled positive for apoptosis more often than hepatocytes in both MPXV groups.
These findings allow for further characterization of differences between MPXV clade pathogenesis, including identifying sites that are important during early viral replication and cellular response to viral infection.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hutson, Christina L.& Carroll, Darin S.& Gallardo-Romero, Nadia& Drew, Clifton& Zaki, Sherif R.& Nagy, Tamas…[et al.]. 2015. Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design. BioMed Research International،Vol. 2015, no. 2015, pp.1-19.
https://search.emarefa.net/detail/BIM-1057413
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hutson, Christina L.…[et al.]. Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design. BioMed Research International No. 2015 (2015), pp.1-19.
https://search.emarefa.net/detail/BIM-1057413
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hutson, Christina L.& Carroll, Darin S.& Gallardo-Romero, Nadia& Drew, Clifton& Zaki, Sherif R.& Nagy, Tamas…[et al.]. Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-19.
https://search.emarefa.net/detail/BIM-1057413
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1057413
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر