Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design
Joint Authors
Hutson, Christina L.
Carroll, Darin S.
Gallardo-Romero, Nadia
Drew, Clifton
Nagy, Tamas
Hughes, Christine
Olson, Victoria A.
Sanders, Jeanine
Patel, Nishi
Smith, Scott K.
Karem, Kevin
Damon, Inger K.
Zaki, Sherif R.
Keckler, M. Shannon
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-24
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Monkeypox virus (MPXV) infection of the prairie dog is valuable to studying systemic orthopoxvirus disease.
To further characterize differences in MPXV clade pathogenesis, groups of prairie dogs were intranasally infected (8×103 p.f.u.) with Congo Basin (CB) or West African (WA) MPXV, and 28 tissues were harvested on days 2, 4, 6, 9, 12, 17, and 24 postinfection.
Samples were evaluated for the presence of virus and gross and microscopic lesions.
Virus was recovered from nasal mucosa, oropharyngeal lymph nodes, and spleen earlier in CB challenged animals (day 4) than WA challenged animals (day 6).
For both groups, primary viremia (indicated by viral DNA) was seen on days 6–9 through day 17.
CB MPXV spread more rapidly, accumulated to greater levels, and caused greater morbidity in animals compared to WA MPXV.
Histopathology and immunohistochemistry (IHC) findings, however, were similar.
Two animals that succumbed to disease demonstrated abundant viral antigen in all organs tested, except for brain.
Dual-IHC staining of select liver and spleen sections showed that apoptotic cells (identified by TUNEL) tended to colocalize with poxvirus antigen.
Interestingly splenocytes were labelled positive for apoptosis more often than hepatocytes in both MPXV groups.
These findings allow for further characterization of differences between MPXV clade pathogenesis, including identifying sites that are important during early viral replication and cellular response to viral infection.
American Psychological Association (APA)
Hutson, Christina L.& Carroll, Darin S.& Gallardo-Romero, Nadia& Drew, Clifton& Zaki, Sherif R.& Nagy, Tamas…[et al.]. 2015. Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design. BioMed Research International،Vol. 2015, no. 2015, pp.1-19.
https://search.emarefa.net/detail/BIM-1057413
Modern Language Association (MLA)
Hutson, Christina L.…[et al.]. Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design. BioMed Research International No. 2015 (2015), pp.1-19.
https://search.emarefa.net/detail/BIM-1057413
American Medical Association (AMA)
Hutson, Christina L.& Carroll, Darin S.& Gallardo-Romero, Nadia& Drew, Clifton& Zaki, Sherif R.& Nagy, Tamas…[et al.]. Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-19.
https://search.emarefa.net/detail/BIM-1057413
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1057413