Identification of Circulating miRNAs in a Mouse Model of Nerve Allograft Transplantation under FK506 Immunosuppression by Illumina Small RNA Deep Sequencing
المؤلفون المشاركون
Yang, Johnson Chia-Shen
Wu, Shao-Chun
Rau, Cheng-Shyuan
Chen, Yi-Chun
Lu, Tsu-Hsiang
Wu, Yi-Chan
Tzeng, Siou-Ling
Wu, Chia-Jung
Hsieh, Ching-Hua
Lin, Chia-Wei
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-09-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
This study aimed to establish the expression profile of circulating microRNAs (miRNAs) during nerve allotransplantation in the presence and absence of FK506 immunosuppression.
Methods.
A 1 cm BALB/c donor sciatic nerve graft was transplanted into the sciatic nerve gaps created in recipient C57BL/6 mice with or without daily FK506 immunosuppression [1 mg/(kg·d)].
At 3, 7, and 14 d after nerve allotransplantation, serum samples were collected for miRNA expression analysis by Illumina small RNA deep sequencing.
Results.
Sequence analysis showed that the dominant size of circulating small RNAs after nerve allotransplantation was 22 nucleotides, followed by 23-nucleotide sequences.
Nine upregulated circulating miRNAs (let-7e-5p, miR-101a-3p, miR-151-5p, miR-181a-5p, miR-204-5p, miR-340-5p, miR-381-3p, miR-411-5p, miR-9-5p, and miR-219-2-3p) were identified at 3 d, but none was identified at 7 or 14 d.
Among them, miR-9-5p had the highest fold-change of >50-fold, followed by miR-340-5p with 38.8-fold.
The presence of these nine miRNAs was not significant at 7 and 14 d after nerve allotransplantation with or without immunosuppression, showing that these miRNAs are not ideal biomarkers for monitoring rejection of deep-buried nerve allografts, a response usually observed later.
Conclusions.
We identified nine upregulated circulating miRNAs, which may have a biological function, particularly during the early stages after nerve allotransplantation under FK506 immunosuppression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Shao-Chun& Rau, Cheng-Shyuan& Yang, Johnson Chia-Shen& Lu, Tsu-Hsiang& Wu, Yi-Chan& Chen, Yi-Chun…[et al.]. 2015. Identification of Circulating miRNAs in a Mouse Model of Nerve Allograft Transplantation under FK506 Immunosuppression by Illumina Small RNA Deep Sequencing. Disease Markers،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061067
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Shao-Chun…[et al.]. Identification of Circulating miRNAs in a Mouse Model of Nerve Allograft Transplantation under FK506 Immunosuppression by Illumina Small RNA Deep Sequencing. Disease Markers No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1061067
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Shao-Chun& Rau, Cheng-Shyuan& Yang, Johnson Chia-Shen& Lu, Tsu-Hsiang& Wu, Yi-Chan& Chen, Yi-Chun…[et al.]. Identification of Circulating miRNAs in a Mouse Model of Nerve Allograft Transplantation under FK506 Immunosuppression by Illumina Small RNA Deep Sequencing. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061067
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1061067
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر