Identification of Circulating miRNAs in a Mouse Model of Nerve Allograft Transplantation under FK506 Immunosuppression by Illumina Small RNA Deep Sequencing
Joint Authors
Yang, Johnson Chia-Shen
Wu, Shao-Chun
Rau, Cheng-Shyuan
Chen, Yi-Chun
Lu, Tsu-Hsiang
Wu, Yi-Chan
Tzeng, Siou-Ling
Wu, Chia-Jung
Hsieh, Ching-Hua
Lin, Chia-Wei
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
This study aimed to establish the expression profile of circulating microRNAs (miRNAs) during nerve allotransplantation in the presence and absence of FK506 immunosuppression.
Methods.
A 1 cm BALB/c donor sciatic nerve graft was transplanted into the sciatic nerve gaps created in recipient C57BL/6 mice with or without daily FK506 immunosuppression [1 mg/(kg·d)].
At 3, 7, and 14 d after nerve allotransplantation, serum samples were collected for miRNA expression analysis by Illumina small RNA deep sequencing.
Results.
Sequence analysis showed that the dominant size of circulating small RNAs after nerve allotransplantation was 22 nucleotides, followed by 23-nucleotide sequences.
Nine upregulated circulating miRNAs (let-7e-5p, miR-101a-3p, miR-151-5p, miR-181a-5p, miR-204-5p, miR-340-5p, miR-381-3p, miR-411-5p, miR-9-5p, and miR-219-2-3p) were identified at 3 d, but none was identified at 7 or 14 d.
Among them, miR-9-5p had the highest fold-change of >50-fold, followed by miR-340-5p with 38.8-fold.
The presence of these nine miRNAs was not significant at 7 and 14 d after nerve allotransplantation with or without immunosuppression, showing that these miRNAs are not ideal biomarkers for monitoring rejection of deep-buried nerve allografts, a response usually observed later.
Conclusions.
We identified nine upregulated circulating miRNAs, which may have a biological function, particularly during the early stages after nerve allotransplantation under FK506 immunosuppression.
American Psychological Association (APA)
Wu, Shao-Chun& Rau, Cheng-Shyuan& Yang, Johnson Chia-Shen& Lu, Tsu-Hsiang& Wu, Yi-Chan& Chen, Yi-Chun…[et al.]. 2015. Identification of Circulating miRNAs in a Mouse Model of Nerve Allograft Transplantation under FK506 Immunosuppression by Illumina Small RNA Deep Sequencing. Disease Markers،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061067
Modern Language Association (MLA)
Wu, Shao-Chun…[et al.]. Identification of Circulating miRNAs in a Mouse Model of Nerve Allograft Transplantation under FK506 Immunosuppression by Illumina Small RNA Deep Sequencing. Disease Markers No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1061067
American Medical Association (AMA)
Wu, Shao-Chun& Rau, Cheng-Shyuan& Yang, Johnson Chia-Shen& Lu, Tsu-Hsiang& Wu, Yi-Chan& Chen, Yi-Chun…[et al.]. Identification of Circulating miRNAs in a Mouse Model of Nerve Allograft Transplantation under FK506 Immunosuppression by Illumina Small RNA Deep Sequencing. Disease Markers. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1061067
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061067