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Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins
المؤلفون المشاركون
Shi, Meina
Liu, Yingting
Feng, Lixing
Cui, Yingbo
Chen, Yajuan
Wang, Peng
Wu, Wenjuan
Chen, Chen
Liu, Xuan
Yang, Weimin
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-18
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Scutellarin (SCU) is one of the main components of traditional Chinese medicine plant Erigeron breviscapus (Vant.) Hand.-Mazz.
In this paper, we studied the protective effects of SCU on human cardiac microvascular endothelial cells (HCMECs) against hypoxia-reoxygenation (HR) injury and its possible target-related proteins.
Results of MTT assay showed that pretreatment of SCU at doses of 1, 5, and 10 μM for 2 h could significantly inhibit the decrease in cell viability of HCMECs induced by HR injury.
Subcellular fractions of cells treated with vehicle control, 1 μM SCU, HR injury, or 1 μM SCU + HR injury were separated by ultracentrifugation.
The protein expression profiles of cytoplasm and membrane/nuclei fractions were checked using protein two-dimensional electrophoresis (2-DE).
Proteins differentially expressed between control and SCU-treated group, control and HR group, or HR and SCU + HR group were identified using mass spectrometry (MS/MS).
Possible interaction network of these target-related proteins was predicted using bioinformatic analysis.
The influence of SCU on the expression levels of these proteins was confirmed using Western blotting assay.
The results indicated that proteins such as p27BBP protein (EIF6), heat shock 60 kDa protein 1 (HSPD1), and chaperonin containing TCP1 subunit 6A isoform (CCT6A) might play important roles in the effects of SCU.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shi, Meina& Liu, Yingting& Feng, Lixing& Cui, Yingbo& Chen, Yajuan& Wang, Peng…[et al.]. 2015. Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1061309
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shi, Meina…[et al.]. Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1061309
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shi, Meina& Liu, Yingting& Feng, Lixing& Cui, Yingbo& Chen, Yajuan& Wang, Peng…[et al.]. Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1061309
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1061309
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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