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Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins
Joint Authors
Shi, Meina
Liu, Yingting
Feng, Lixing
Cui, Yingbo
Chen, Yajuan
Wang, Peng
Wu, Wenjuan
Chen, Chen
Liu, Xuan
Yang, Weimin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-18
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Scutellarin (SCU) is one of the main components of traditional Chinese medicine plant Erigeron breviscapus (Vant.) Hand.-Mazz.
In this paper, we studied the protective effects of SCU on human cardiac microvascular endothelial cells (HCMECs) against hypoxia-reoxygenation (HR) injury and its possible target-related proteins.
Results of MTT assay showed that pretreatment of SCU at doses of 1, 5, and 10 μM for 2 h could significantly inhibit the decrease in cell viability of HCMECs induced by HR injury.
Subcellular fractions of cells treated with vehicle control, 1 μM SCU, HR injury, or 1 μM SCU + HR injury were separated by ultracentrifugation.
The protein expression profiles of cytoplasm and membrane/nuclei fractions were checked using protein two-dimensional electrophoresis (2-DE).
Proteins differentially expressed between control and SCU-treated group, control and HR group, or HR and SCU + HR group were identified using mass spectrometry (MS/MS).
Possible interaction network of these target-related proteins was predicted using bioinformatic analysis.
The influence of SCU on the expression levels of these proteins was confirmed using Western blotting assay.
The results indicated that proteins such as p27BBP protein (EIF6), heat shock 60 kDa protein 1 (HSPD1), and chaperonin containing TCP1 subunit 6A isoform (CCT6A) might play important roles in the effects of SCU.
American Psychological Association (APA)
Shi, Meina& Liu, Yingting& Feng, Lixing& Cui, Yingbo& Chen, Yajuan& Wang, Peng…[et al.]. 2015. Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1061309
Modern Language Association (MLA)
Shi, Meina…[et al.]. Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1061309
American Medical Association (AMA)
Shi, Meina& Liu, Yingting& Feng, Lixing& Cui, Yingbo& Chen, Yajuan& Wang, Peng…[et al.]. Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1061309
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1061309