Shikonin Inhibits Inflammatory Response in Rheumatoid Arthritis Synovial Fibroblasts via lncRNA-NR024118
المؤلفون المشاركون
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-10
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Background.
Shikonin is a major chemical component of zicao that possesses anti-inflammatory properties and the ability to mediate cellular and humoral immunity, especially in rheumatoid arthritis (RA).
We investigated the impact of shikonin on inflammatory response in RA synovial fibroblasts using the CAIA model.
Methods.
Severe polyarticular arthritis was induced in Balb/c female mice.
Expressions of lncRNA-NR024118, SOCS3, proinflammatory cytokines, and MMPs were evaluated using RT-RCR.
Histone acetylation and SOCS3 protein expression were assessed by ChIP assay and western blot, respectively.
Results.
Mice treated with shikonin showed an abrogation of soft tissue and bone lesions.
Shikonin remarkably enhanced the expression of NR024118 and SOCS3 and suppressed the secretion and expression of IL-6, IL-8, and MMPs.
Proliferation of cultured RA synovial fibroblasts in the presence of IL-1β was also significantly inhibited by shikonin.
Moreover, shikonin dose-dependently increased acetylation of histone H3 at the promoter of NR024118.
Finally, NR024118 overexpression and interference significantly changed SOCS3 expression and NR024118 interference could reverse regulation of shikonin on SOCS3, proinflammatory cytokines, and MMPs expression level in MH7A cells.
Conclusion.
Our results reveal that, in the CAIA mouse model of RA, shikonin has disease modifying activity that is attributable to the inhibition of inflammatory response via lncRNA-NR024118.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Ke-ya& Chen, Dong-liang. 2015. Shikonin Inhibits Inflammatory Response in Rheumatoid Arthritis Synovial Fibroblasts via lncRNA-NR024118. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063407
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Ke-ya& Chen, Dong-liang. Shikonin Inhibits Inflammatory Response in Rheumatoid Arthritis Synovial Fibroblasts via lncRNA-NR024118. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1063407
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Ke-ya& Chen, Dong-liang. Shikonin Inhibits Inflammatory Response in Rheumatoid Arthritis Synovial Fibroblasts via lncRNA-NR024118. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063407
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1063407
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر