Shikonin Inhibits Inflammatory Response in Rheumatoid Arthritis Synovial Fibroblasts via lncRNA-NR024118
Joint Authors
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Shikonin is a major chemical component of zicao that possesses anti-inflammatory properties and the ability to mediate cellular and humoral immunity, especially in rheumatoid arthritis (RA).
We investigated the impact of shikonin on inflammatory response in RA synovial fibroblasts using the CAIA model.
Methods.
Severe polyarticular arthritis was induced in Balb/c female mice.
Expressions of lncRNA-NR024118, SOCS3, proinflammatory cytokines, and MMPs were evaluated using RT-RCR.
Histone acetylation and SOCS3 protein expression were assessed by ChIP assay and western blot, respectively.
Results.
Mice treated with shikonin showed an abrogation of soft tissue and bone lesions.
Shikonin remarkably enhanced the expression of NR024118 and SOCS3 and suppressed the secretion and expression of IL-6, IL-8, and MMPs.
Proliferation of cultured RA synovial fibroblasts in the presence of IL-1β was also significantly inhibited by shikonin.
Moreover, shikonin dose-dependently increased acetylation of histone H3 at the promoter of NR024118.
Finally, NR024118 overexpression and interference significantly changed SOCS3 expression and NR024118 interference could reverse regulation of shikonin on SOCS3, proinflammatory cytokines, and MMPs expression level in MH7A cells.
Conclusion.
Our results reveal that, in the CAIA mouse model of RA, shikonin has disease modifying activity that is attributable to the inhibition of inflammatory response via lncRNA-NR024118.
American Psychological Association (APA)
Yang, Ke-ya& Chen, Dong-liang. 2015. Shikonin Inhibits Inflammatory Response in Rheumatoid Arthritis Synovial Fibroblasts via lncRNA-NR024118. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063407
Modern Language Association (MLA)
Yang, Ke-ya& Chen, Dong-liang. Shikonin Inhibits Inflammatory Response in Rheumatoid Arthritis Synovial Fibroblasts via lncRNA-NR024118. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1063407
American Medical Association (AMA)
Yang, Ke-ya& Chen, Dong-liang. Shikonin Inhibits Inflammatory Response in Rheumatoid Arthritis Synovial Fibroblasts via lncRNA-NR024118. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1063407
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063407