Effect of Rat Medicated Serum Containing Zuo Gui Wan andor You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro
المؤلفون المشاركون
Deng, Yan-li
Lu, Hua
Hu, Xiang
Yie, Shang-mian
Wan, Qian
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-01-22
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Zuo Gui Wan (ZGW) and You Gui Wan (YGW) are two classic formulas used in clinical treatment of infertility in traditional Chinese medicine (TCM).
However, the actions of the formulas remain to be proven at the cellular and molecular levels.
In this study, we investigate whether the two formulas have any effect on germ cell formation and differentiation by culturing rat medicated serums containing YGW or ZGW with stem cells derived from human first trimester umbilical cord.
Our results showed that while the normal rat serums had no significant effects, the rat medicated serums had significant effects on the differentiation of the stem cells into oocyte-like cells (OLCs) based on (1) cell morphological changes that resembled purative cumulus-oocyte complexes (COCs); (2) expressions of specific markers that were indicative of germ cell formation and oocyte development; and (3) estradiol production by the COC-like cells.
Furthermore, ZGW medicated serums exhibited more obvious effects on specific gene expressions of germ cells, whereas YGW medicated serums showed stronger effects on estradiol production.
Accordingly, our study provides evidence demonstrating for the first time that one of molecular and cellular actions of YGW or ZGW in treating human reproductive dysfunctions may be through an enhancement of neooogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hu, Xiang& Lu, Hua& Deng, Yan-li& Wan, Qian& Yie, Shang-mian. 2015. Effect of Rat Medicated Serum Containing Zuo Gui Wan andor You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1063653
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hu, Xiang…[et al.]. Effect of Rat Medicated Serum Containing Zuo Gui Wan andor You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1063653
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hu, Xiang& Lu, Hua& Deng, Yan-li& Wan, Qian& Yie, Shang-mian. Effect of Rat Medicated Serum Containing Zuo Gui Wan andor You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1063653
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1063653
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر