Effect of Rat Medicated Serum Containing Zuo Gui Wan andor You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro
Joint Authors
Deng, Yan-li
Lu, Hua
Hu, Xiang
Yie, Shang-mian
Wan, Qian
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-22
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Zuo Gui Wan (ZGW) and You Gui Wan (YGW) are two classic formulas used in clinical treatment of infertility in traditional Chinese medicine (TCM).
However, the actions of the formulas remain to be proven at the cellular and molecular levels.
In this study, we investigate whether the two formulas have any effect on germ cell formation and differentiation by culturing rat medicated serums containing YGW or ZGW with stem cells derived from human first trimester umbilical cord.
Our results showed that while the normal rat serums had no significant effects, the rat medicated serums had significant effects on the differentiation of the stem cells into oocyte-like cells (OLCs) based on (1) cell morphological changes that resembled purative cumulus-oocyte complexes (COCs); (2) expressions of specific markers that were indicative of germ cell formation and oocyte development; and (3) estradiol production by the COC-like cells.
Furthermore, ZGW medicated serums exhibited more obvious effects on specific gene expressions of germ cells, whereas YGW medicated serums showed stronger effects on estradiol production.
Accordingly, our study provides evidence demonstrating for the first time that one of molecular and cellular actions of YGW or ZGW in treating human reproductive dysfunctions may be through an enhancement of neooogenesis.
American Psychological Association (APA)
Hu, Xiang& Lu, Hua& Deng, Yan-li& Wan, Qian& Yie, Shang-mian. 2015. Effect of Rat Medicated Serum Containing Zuo Gui Wan andor You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1063653
Modern Language Association (MLA)
Hu, Xiang…[et al.]. Effect of Rat Medicated Serum Containing Zuo Gui Wan andor You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1063653
American Medical Association (AMA)
Hu, Xiang& Lu, Hua& Deng, Yan-li& Wan, Qian& Yie, Shang-mian. Effect of Rat Medicated Serum Containing Zuo Gui Wan andor You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1063653
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1063653