Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia

الملخص EN

The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis.

The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods.

Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L.

borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay.

The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively.

The GC-MS analysis revealed the presence of at least 35 compounds.

The extract was found to induce cytotoxicity against MCF-7 cell line with IC50 value of 47.33 ± 7.37 µg/mL.

Cell cycle analysis showed that the extract induced significant arrest at G0/G1 at 24 hours of treatment.

After 72 hours of treatment, the proportion of cells in G0/G1 and G2-M phases had decreased significantly as compared to their control.

Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment.

No activation of caspase 3 was observed.

These findings suggest that L.

borneensis extract has the potential as natural antioxidant and anticancer agents.