![](/images/graphics-bg.png)
Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation
المؤلفون المشاركون
Wang, Jigang
Lee, Yew Mun
Li, Caixia
Li, Ping
Li, Zhen
Lim, Teck Kwang
Gong, Zhiyuan
Lin, Qingsong
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-19
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Majority of the proteomic studies on tissue samples involve the use of gel-based approach for profiling and digestion.
The laborious gel-based approach is slowly being replaced by the advancing in-solution digestion approach.
However, there are still several difficulties such as difficult-to-solubilize proteins, poor proteomic analysis in complex tissue samples, and the presence of sample impurities.
Henceforth, there is a great demand to formulate a highly efficient protein extraction buffer with high protein extraction efficiency from tissue samples, high compatibility with in-solution digestion, reduced number of sample handling steps to reduce sample loss, low time consumption, low cost, and ease of usage.
Here, we evaluated various existing protein extraction buffers with zebrafish liver tumor samples and found that sodium deoxycholate- (DOC-) based extraction buffer with heat denaturation was the most effective approach for highly efficient extraction of proteins from complex tissues such as the zebrafish liver tumor.
A total of 4,790 proteins have been identified using shotgun proteomics approach with 2D LC, which to our knowledge is the most comprehensive study for zebrafish liver tumor proteome.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Jigang& Lee, Yew Mun& Li, Caixia& Li, Ping& Li, Zhen& Lim, Teck Kwang…[et al.]. 2015. Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1064441
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Jigang…[et al.]. Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1064441
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Jigang& Lee, Yew Mun& Li, Caixia& Li, Ping& Li, Zhen& Lim, Teck Kwang…[et al.]. Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1064441
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1064441
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)