Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation
Joint Authors
Wang, Jigang
Lee, Yew Mun
Li, Caixia
Li, Ping
Li, Zhen
Lim, Teck Kwang
Gong, Zhiyuan
Lin, Qingsong
Source
International Journal of Analytical Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Majority of the proteomic studies on tissue samples involve the use of gel-based approach for profiling and digestion.
The laborious gel-based approach is slowly being replaced by the advancing in-solution digestion approach.
However, there are still several difficulties such as difficult-to-solubilize proteins, poor proteomic analysis in complex tissue samples, and the presence of sample impurities.
Henceforth, there is a great demand to formulate a highly efficient protein extraction buffer with high protein extraction efficiency from tissue samples, high compatibility with in-solution digestion, reduced number of sample handling steps to reduce sample loss, low time consumption, low cost, and ease of usage.
Here, we evaluated various existing protein extraction buffers with zebrafish liver tumor samples and found that sodium deoxycholate- (DOC-) based extraction buffer with heat denaturation was the most effective approach for highly efficient extraction of proteins from complex tissues such as the zebrafish liver tumor.
A total of 4,790 proteins have been identified using shotgun proteomics approach with 2D LC, which to our knowledge is the most comprehensive study for zebrafish liver tumor proteome.
American Psychological Association (APA)
Wang, Jigang& Lee, Yew Mun& Li, Caixia& Li, Ping& Li, Zhen& Lim, Teck Kwang…[et al.]. 2015. Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation. International Journal of Analytical Chemistry،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1064441
Modern Language Association (MLA)
Wang, Jigang…[et al.]. Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation. International Journal of Analytical Chemistry No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1064441
American Medical Association (AMA)
Wang, Jigang& Lee, Yew Mun& Li, Caixia& Li, Ping& Li, Zhen& Lim, Teck Kwang…[et al.]. Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation. International Journal of Analytical Chemistry. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1064441
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1064441