Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations

المؤلفون المشاركون

Lange, R.
Bergemann, Claudia
Nebe, J. Barbara
Elter, Patrick
Weißmann, Volker
Hansmann, Harald
Klinkenberg, Ernst-Dieter

المصدر

International Journal of Biomaterials

العدد

المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2015-10-11

دولة النشر

مصر

عدد الصفحات

12

التخصصات الرئيسية

الطب البشري

الملخص EN

Studies on bone cell ingrowth into synthetic, porous three-dimensional (3D) implants showed difficulties arising from impaired cellular proliferation and differentiation in the core region of these scaffolds with increasing scaffold volume in vitro.

Therefore, we developed an in vitro perfusion cell culture module, which allows the analysis of cells in the interior of scaffolds under different medium flow rates.

For each flow rate the cell viability was measured and compared with results from computer simulations that predict the local oxygen supply and shear stress inside the scaffold based on the finite element method.

We found that the local cell viability correlates with the local oxygen concentration and the local shear stress.

On the one hand the oxygen supply of the cells in the core becomes optimal with a higher perfusion flow.

On the other hand shear stress caused by high flow rates impedes cell vitality, especially at the surface of the scaffold.

Our results demonstrate that both parameters must be considered to derive an optimal nutrient flow rate.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Bergemann, Claudia& Elter, Patrick& Lange, R.& Weißmann, Volker& Hansmann, Harald& Klinkenberg, Ernst-Dieter…[et al.]. 2015. Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations. International Journal of Biomaterials،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1065301

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Bergemann, Claudia…[et al.]. Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations. International Journal of Biomaterials No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1065301

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Bergemann, Claudia& Elter, Patrick& Lange, R.& Weißmann, Volker& Hansmann, Harald& Klinkenberg, Ernst-Dieter…[et al.]. Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations. International Journal of Biomaterials. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1065301

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1065301