Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations
Joint Authors
Lange, R.
Bergemann, Claudia
Nebe, J. Barbara
Elter, Patrick
Weißmann, Volker
Hansmann, Harald
Klinkenberg, Ernst-Dieter
Source
International Journal of Biomaterials
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Studies on bone cell ingrowth into synthetic, porous three-dimensional (3D) implants showed difficulties arising from impaired cellular proliferation and differentiation in the core region of these scaffolds with increasing scaffold volume in vitro.
Therefore, we developed an in vitro perfusion cell culture module, which allows the analysis of cells in the interior of scaffolds under different medium flow rates.
For each flow rate the cell viability was measured and compared with results from computer simulations that predict the local oxygen supply and shear stress inside the scaffold based on the finite element method.
We found that the local cell viability correlates with the local oxygen concentration and the local shear stress.
On the one hand the oxygen supply of the cells in the core becomes optimal with a higher perfusion flow.
On the other hand shear stress caused by high flow rates impedes cell vitality, especially at the surface of the scaffold.
Our results demonstrate that both parameters must be considered to derive an optimal nutrient flow rate.
American Psychological Association (APA)
Bergemann, Claudia& Elter, Patrick& Lange, R.& Weißmann, Volker& Hansmann, Harald& Klinkenberg, Ernst-Dieter…[et al.]. 2015. Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations. International Journal of Biomaterials،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1065301
Modern Language Association (MLA)
Bergemann, Claudia…[et al.]. Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations. International Journal of Biomaterials No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1065301
American Medical Association (AMA)
Bergemann, Claudia& Elter, Patrick& Lange, R.& Weißmann, Volker& Hansmann, Harald& Klinkenberg, Ernst-Dieter…[et al.]. Cellular Nutrition in Complex Three-Dimensional Scaffolds: A Comparison between Experiments and Computer Simulations. International Journal of Biomaterials. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1065301
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065301