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Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-Dimensional In Vitro Model of Periodontium
المؤلفون المشاركون
Berahim, Zurairah
Jowett, Adrian K.
Rawlinson, Andrew
Moharamzadeh, Keyvan
المصدر
International Journal of Dentistry
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-08
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The aim of this study was to develop a three-dimensional in vitro model of periodontium to investigate the osteogenic and cementogenic differentiation potential of the periodontal ligament fibroblast (PDLF) spheroids within a dentin-membrane complex.
PDLFs were cultured in both spheroid forms and monolayers and were seeded onto two biological collagen-based and synthetic membranes.
Cell-membrane composites were then transferred onto dentin slices with fibroblasts facing the dentin surface and further cultured for 20 days.
The composites were then processed for histology and immunohistochemical analyses for osteocalcin, Runx2, periostin, and cementum attachment protein (CAP).
Both membranes seeded with PDLF-derived cells adhered to dentin and fibroblasts were present at the dentin interface and spread within both membranes.
All membrane-cell-dentine composites showed positive staining for osteocalcin, Runx2, and periostin.
However, CAP was not expressed by any of the tissue composites.
It can be concluded that PDLFs exhibited some osteogenic potential when cultured in a 3D matrix in the presence of dentin as shown by the expression of osteocalcin.
However the interaction of cells and dentin in this study was unable to stimulate cementum formation.
The type of membrane did not have a significant effect upon differentiation, but fibroblast seeded-PGA membrane demonstrated better attachment to dentin than the collagen membrane.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Berahim, Zurairah& Moharamzadeh, Keyvan& Jowett, Adrian K.& Rawlinson, Andrew. 2015. Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-Dimensional In Vitro Model of Periodontium. International Journal of Dentistry،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1065477
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Berahim, Zurairah…[et al.]. Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-Dimensional In Vitro Model of Periodontium. International Journal of Dentistry No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1065477
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Berahim, Zurairah& Moharamzadeh, Keyvan& Jowett, Adrian K.& Rawlinson, Andrew. Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-Dimensional In Vitro Model of Periodontium. International Journal of Dentistry. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1065477
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1065477
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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