Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-Dimensional In Vitro Model of Periodontium
Joint Authors
Berahim, Zurairah
Jowett, Adrian K.
Rawlinson, Andrew
Moharamzadeh, Keyvan
Source
International Journal of Dentistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The aim of this study was to develop a three-dimensional in vitro model of periodontium to investigate the osteogenic and cementogenic differentiation potential of the periodontal ligament fibroblast (PDLF) spheroids within a dentin-membrane complex.
PDLFs were cultured in both spheroid forms and monolayers and were seeded onto two biological collagen-based and synthetic membranes.
Cell-membrane composites were then transferred onto dentin slices with fibroblasts facing the dentin surface and further cultured for 20 days.
The composites were then processed for histology and immunohistochemical analyses for osteocalcin, Runx2, periostin, and cementum attachment protein (CAP).
Both membranes seeded with PDLF-derived cells adhered to dentin and fibroblasts were present at the dentin interface and spread within both membranes.
All membrane-cell-dentine composites showed positive staining for osteocalcin, Runx2, and periostin.
However, CAP was not expressed by any of the tissue composites.
It can be concluded that PDLFs exhibited some osteogenic potential when cultured in a 3D matrix in the presence of dentin as shown by the expression of osteocalcin.
However the interaction of cells and dentin in this study was unable to stimulate cementum formation.
The type of membrane did not have a significant effect upon differentiation, but fibroblast seeded-PGA membrane demonstrated better attachment to dentin than the collagen membrane.
American Psychological Association (APA)
Berahim, Zurairah& Moharamzadeh, Keyvan& Jowett, Adrian K.& Rawlinson, Andrew. 2015. Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-Dimensional In Vitro Model of Periodontium. International Journal of Dentistry،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1065477
Modern Language Association (MLA)
Berahim, Zurairah…[et al.]. Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-Dimensional In Vitro Model of Periodontium. International Journal of Dentistry No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1065477
American Medical Association (AMA)
Berahim, Zurairah& Moharamzadeh, Keyvan& Jowett, Adrian K.& Rawlinson, Andrew. Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-Dimensional In Vitro Model of Periodontium. International Journal of Dentistry. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1065477
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1065477