Revealing Genomic Profile That Underlies Tropism of Myeloma Cells Using Whole Exome Sequencing
المؤلفون المشاركون
Jung, Woo June
Ahn, Kwang-Sung
Koh, Youngil
Kim, Daeyoon
Yoon, Sung-Soo
المصدر
International Journal of Genomics
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-18
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background.
Previously we established two cell lines (SNU_MM1393_BM and SNU_MM1393_SC) from different tissues (bone marrow and subcutis) of mice which were injected with single patient’s myeloma sample.
We tried to define genetic changes specific for each cell line using whole exome sequencing (WES).
Materials and Methods.
We extracted DNA from SNU_MM1393_BM and SNU_MM1393_SC and performed WES.
For single nucleotide variants (SNV) calling, we used Varscan2.
Annotation of mutation was performed using ANNOVAR.
Results.
When calling of somatic mutations was performed, 68 genes were nonsynonymously mutated only in SNU_MM1393_SC, while 136 genes were nonsynonymously mutated only in SNU_MM1393_BM.
KIAA1199, FRY, AP3B2, and OPTC were representative genes specifically mutated in SNU_MM1393_SC.
When comparison analysis was performed using TCGA data, mutational pattern of SNU_MM1393_SC resembled that of melanoma mostly.
Pathway analysis using KEGG database showed that mutated genes specific of SNU_MM1393_BM were related to differentiation, while those of SNU_MM1393_SC were related to tumorigenesis.
Conclusion.
We found out genetic changes that underlie tropism of myeloma cells using WES.
Genetic signature of cutaneous plasmacytoma shares that of melanoma implying common mechanism for skin tropism.
KIAA1199, FRY, AP3B2, and OPTC are candidate genes for skin tropism of cancers.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Koh, Youngil& Kim, Daeyoon& Jung, Woo June& Ahn, Kwang-Sung& Yoon, Sung-Soo. 2015. Revealing Genomic Profile That Underlies Tropism of Myeloma Cells Using Whole Exome Sequencing. International Journal of Genomics،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1066035
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Koh, Youngil…[et al.]. Revealing Genomic Profile That Underlies Tropism of Myeloma Cells Using Whole Exome Sequencing. International Journal of Genomics No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1066035
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Koh, Youngil& Kim, Daeyoon& Jung, Woo June& Ahn, Kwang-Sung& Yoon, Sung-Soo. Revealing Genomic Profile That Underlies Tropism of Myeloma Cells Using Whole Exome Sequencing. International Journal of Genomics. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1066035
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1066035
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر