Revealing Genomic Profile That Underlies Tropism of Myeloma Cells Using Whole Exome Sequencing
Joint Authors
Jung, Woo June
Ahn, Kwang-Sung
Koh, Youngil
Kim, Daeyoon
Yoon, Sung-Soo
Source
International Journal of Genomics
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Previously we established two cell lines (SNU_MM1393_BM and SNU_MM1393_SC) from different tissues (bone marrow and subcutis) of mice which were injected with single patient’s myeloma sample.
We tried to define genetic changes specific for each cell line using whole exome sequencing (WES).
Materials and Methods.
We extracted DNA from SNU_MM1393_BM and SNU_MM1393_SC and performed WES.
For single nucleotide variants (SNV) calling, we used Varscan2.
Annotation of mutation was performed using ANNOVAR.
Results.
When calling of somatic mutations was performed, 68 genes were nonsynonymously mutated only in SNU_MM1393_SC, while 136 genes were nonsynonymously mutated only in SNU_MM1393_BM.
KIAA1199, FRY, AP3B2, and OPTC were representative genes specifically mutated in SNU_MM1393_SC.
When comparison analysis was performed using TCGA data, mutational pattern of SNU_MM1393_SC resembled that of melanoma mostly.
Pathway analysis using KEGG database showed that mutated genes specific of SNU_MM1393_BM were related to differentiation, while those of SNU_MM1393_SC were related to tumorigenesis.
Conclusion.
We found out genetic changes that underlie tropism of myeloma cells using WES.
Genetic signature of cutaneous plasmacytoma shares that of melanoma implying common mechanism for skin tropism.
KIAA1199, FRY, AP3B2, and OPTC are candidate genes for skin tropism of cancers.
American Psychological Association (APA)
Koh, Youngil& Kim, Daeyoon& Jung, Woo June& Ahn, Kwang-Sung& Yoon, Sung-Soo. 2015. Revealing Genomic Profile That Underlies Tropism of Myeloma Cells Using Whole Exome Sequencing. International Journal of Genomics،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1066035
Modern Language Association (MLA)
Koh, Youngil…[et al.]. Revealing Genomic Profile That Underlies Tropism of Myeloma Cells Using Whole Exome Sequencing. International Journal of Genomics No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1066035
American Medical Association (AMA)
Koh, Youngil& Kim, Daeyoon& Jung, Woo June& Ahn, Kwang-Sung& Yoon, Sung-Soo. Revealing Genomic Profile That Underlies Tropism of Myeloma Cells Using Whole Exome Sequencing. International Journal of Genomics. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1066035
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066035