Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening
المؤلفون المشاركون
Gurumurthy, Channabasavaiah B.
Joshi, Poonam S.
Kurz, Scott G.
Quadros, Rolen M.
Harms, Donald W.
Lloyd, K. C. Kent
Ohtsuka, Masato
المصدر
International Journal of Genomics
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-02-28
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Marker assisted speed congenics technique is commonly used to facilitate backcrossing of mouse strains in nearly half the time it normally takes otherwise.
Traditionally, the technique is performed by analyzing PCR amplified regions of simple sequence length polymorphism (SSLP) markers between the recipient and donor strains: offspring with the highest number of markers showing the recipient genome across all chromosomes is chosen for the next generation.
Although there are well-defined panels of SSLP makers established between certain pairs of mice strains, they are incomplete for most strains.
The availability of well-established marker sets for speed congenic screens would enable the scientific community to transfer mutations across strain backgrounds.
In this study, we tested the suitability of over 400 SSLP marker sets among 10 mouse strains commonly used for generating genetically engineered models.
The panel of markers presented here can readily identify the specified strains and will be quite useful in marker assisted speed congenic screens.
Moreover, unlike newer single nucleotide polymorphism (SNP) array methods which require sophisticated equipment, the SSLP markers panel described here only uses PCR and agarose gel electrophoresis of amplified products; therefore it can be performed in most research laboratories.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gurumurthy, Channabasavaiah B.& Joshi, Poonam S.& Kurz, Scott G.& Ohtsuka, Masato& Quadros, Rolen M.& Harms, Donald W.…[et al.]. 2015. Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening. International Journal of Genomics،Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1066040
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gurumurthy, Channabasavaiah B.…[et al.]. Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening. International Journal of Genomics No. 2015 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1066040
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gurumurthy, Channabasavaiah B.& Joshi, Poonam S.& Kurz, Scott G.& Ohtsuka, Masato& Quadros, Rolen M.& Harms, Donald W.…[et al.]. Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening. International Journal of Genomics. 2015. Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1066040
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1066040
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر