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Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening
Joint Authors
Gurumurthy, Channabasavaiah B.
Joshi, Poonam S.
Kurz, Scott G.
Quadros, Rolen M.
Harms, Donald W.
Lloyd, K. C. Kent
Ohtsuka, Masato
Source
International Journal of Genomics
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-28
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Marker assisted speed congenics technique is commonly used to facilitate backcrossing of mouse strains in nearly half the time it normally takes otherwise.
Traditionally, the technique is performed by analyzing PCR amplified regions of simple sequence length polymorphism (SSLP) markers between the recipient and donor strains: offspring with the highest number of markers showing the recipient genome across all chromosomes is chosen for the next generation.
Although there are well-defined panels of SSLP makers established between certain pairs of mice strains, they are incomplete for most strains.
The availability of well-established marker sets for speed congenic screens would enable the scientific community to transfer mutations across strain backgrounds.
In this study, we tested the suitability of over 400 SSLP marker sets among 10 mouse strains commonly used for generating genetically engineered models.
The panel of markers presented here can readily identify the specified strains and will be quite useful in marker assisted speed congenic screens.
Moreover, unlike newer single nucleotide polymorphism (SNP) array methods which require sophisticated equipment, the SSLP markers panel described here only uses PCR and agarose gel electrophoresis of amplified products; therefore it can be performed in most research laboratories.
American Psychological Association (APA)
Gurumurthy, Channabasavaiah B.& Joshi, Poonam S.& Kurz, Scott G.& Ohtsuka, Masato& Quadros, Rolen M.& Harms, Donald W.…[et al.]. 2015. Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening. International Journal of Genomics،Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1066040
Modern Language Association (MLA)
Gurumurthy, Channabasavaiah B.…[et al.]. Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening. International Journal of Genomics No. 2015 (2015), pp.1-17.
https://search.emarefa.net/detail/BIM-1066040
American Medical Association (AMA)
Gurumurthy, Channabasavaiah B.& Joshi, Poonam S.& Kurz, Scott G.& Ohtsuka, Masato& Quadros, Rolen M.& Harms, Donald W.…[et al.]. Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening. International Journal of Genomics. 2015. Vol. 2015, no. 2015, pp.1-17.
https://search.emarefa.net/detail/BIM-1066040
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066040