Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing
المؤلفون المشاركون
Du, Guan-Hua
Qin, Xue-Mei
Tian, Hongling
Xu, Xiaoshuang
Zhang, Fusheng
Wang, Yaoqin
Guo, Shuhong
المصدر
International Journal of Genomics
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-12
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Radix polygalae, the dried roots of Polygala tenuifolia and P.
sibirica, is one of the most well-known traditional Chinese medicinal plants.
Radix polygalae contains various saponins, xanthones, and oligosaccharide esters and these compounds are responsible for several pharmacological properties.
To provide basic breeding information, enhance molecular biological analysis, and determine secondary metabolite biosynthetic pathways of P.
tenuifolia, we applied Illumina sequencing technology and de novo assembly.
We also applied this technique to gain an overview of P.
tenuifolia transcriptome from samples with different years.
Using Illumina sequencing, approximately 67.2% of unique sequences were annotated by basic local alignment search tool similarity searches against public sequence databases.
We classified the annotated unigenes by using Nr, Nt, GO, COG, and KEGG databases compared with NCBI.
We also obtained many candidates CYP450s and UGTs by the analysis of genes in the secondary metabolite biosynthetic pathways, including putative terpenoid backbone and phenylpropanoid biosynthesis pathway.
With this transcriptome sequencing, future genetic and genomics studies related to the molecular mechanisms associated with the chemical composition of P.
tenuifolia may be improved.
Genes involved in the enrichment of secondary metabolite biosynthesis-related pathways could enhance the potential applications of P.
tenuifolia in pharmaceutical industries.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tian, Hongling& Xu, Xiaoshuang& Zhang, Fusheng& Wang, Yaoqin& Guo, Shuhong& Qin, Xue-Mei…[et al.]. 2015. Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing. International Journal of Genomics،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1066044
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tian, Hongling…[et al.]. Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing. International Journal of Genomics No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1066044
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tian, Hongling& Xu, Xiaoshuang& Zhang, Fusheng& Wang, Yaoqin& Guo, Shuhong& Qin, Xue-Mei…[et al.]. Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing. International Journal of Genomics. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1066044
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1066044
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر