Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing
Joint Authors
Du, Guan-Hua
Qin, Xue-Mei
Tian, Hongling
Xu, Xiaoshuang
Zhang, Fusheng
Wang, Yaoqin
Guo, Shuhong
Source
International Journal of Genomics
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Radix polygalae, the dried roots of Polygala tenuifolia and P.
sibirica, is one of the most well-known traditional Chinese medicinal plants.
Radix polygalae contains various saponins, xanthones, and oligosaccharide esters and these compounds are responsible for several pharmacological properties.
To provide basic breeding information, enhance molecular biological analysis, and determine secondary metabolite biosynthetic pathways of P.
tenuifolia, we applied Illumina sequencing technology and de novo assembly.
We also applied this technique to gain an overview of P.
tenuifolia transcriptome from samples with different years.
Using Illumina sequencing, approximately 67.2% of unique sequences were annotated by basic local alignment search tool similarity searches against public sequence databases.
We classified the annotated unigenes by using Nr, Nt, GO, COG, and KEGG databases compared with NCBI.
We also obtained many candidates CYP450s and UGTs by the analysis of genes in the secondary metabolite biosynthetic pathways, including putative terpenoid backbone and phenylpropanoid biosynthesis pathway.
With this transcriptome sequencing, future genetic and genomics studies related to the molecular mechanisms associated with the chemical composition of P.
tenuifolia may be improved.
Genes involved in the enrichment of secondary metabolite biosynthesis-related pathways could enhance the potential applications of P.
tenuifolia in pharmaceutical industries.
American Psychological Association (APA)
Tian, Hongling& Xu, Xiaoshuang& Zhang, Fusheng& Wang, Yaoqin& Guo, Shuhong& Qin, Xue-Mei…[et al.]. 2015. Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing. International Journal of Genomics،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1066044
Modern Language Association (MLA)
Tian, Hongling…[et al.]. Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing. International Journal of Genomics No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1066044
American Medical Association (AMA)
Tian, Hongling& Xu, Xiaoshuang& Zhang, Fusheng& Wang, Yaoqin& Guo, Shuhong& Qin, Xue-Mei…[et al.]. Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing. International Journal of Genomics. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1066044
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066044