Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro
المؤلفون المشاركون
Duerksen-Hughes, Penelope
Chan, Philip J.
Tolen, Jennifer A.
Lau, Kathleen
المصدر
International Journal of Reproductive Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-08-04
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Objectives.
Human papillomaviruses (HPV) are associated with cell cycle arrest.
This study focused on antioxidant selenomethionine (SeMet) inhibition of HPV-mediated necrosis.
The objectives were to determine HPV-18 effects on embryonic cells and to evaluate SeMet in blocking HPV-18 effects.
Methods.
Fertilized mouse embryos were cultured for 5 days to implanted trophoblasts and exposed to either control medium (group 1), HPV-18 (group 2), combined HPV-18 and 0.5 µM SeMet (group 3), or combined HPV-18 and 5.0 µM SeMet (group 4).
After 48 hrs, trophoblast integrity and, apoptosis/necrosis were assessed using morphometric and dual-stain fluorescence assays, respectively.
Results.
HPV-18 exposed trophoblasts nuclei (253.8 ± 28.5 sq·µ) were 29% smaller than controls (355.6 ± 35.9 sq·µ).
Supplementation with 0.5 and 5.0 µM SeMet prevented nuclear shrinkage after HPV-18 exposure.
HPV-18 infected trophoblasts remained larger with SeMet supplementation.
HPV-18 decreased cell viability by 44% but SeMet supplementation sustained cell viability.
Apoptosis was lower when SeMet was present.
HPV-18 decreased inner cell mass (ICM) viability by over 60%.
Conclusions.
HPV-18 decreased nuclear size and trophoblast viability but these effects were attenuated by the antioxidant SeMet.
SeMet blocked HPV-18 associated apoptosis process in trophoblasts but not ICM cells suggesting involvement of different oxidative stress pathways.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tolen, Jennifer A.& Duerksen-Hughes, Penelope& Lau, Kathleen& Chan, Philip J.. 2015. Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro. International Journal of Reproductive Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1066953
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tolen, Jennifer A.…[et al.]. Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro. International Journal of Reproductive Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1066953
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tolen, Jennifer A.& Duerksen-Hughes, Penelope& Lau, Kathleen& Chan, Philip J.. Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro. International Journal of Reproductive Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1066953
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1066953
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر