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Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro
Joint Authors
Duerksen-Hughes, Penelope
Chan, Philip J.
Tolen, Jennifer A.
Lau, Kathleen
Source
International Journal of Reproductive Medicine
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objectives.
Human papillomaviruses (HPV) are associated with cell cycle arrest.
This study focused on antioxidant selenomethionine (SeMet) inhibition of HPV-mediated necrosis.
The objectives were to determine HPV-18 effects on embryonic cells and to evaluate SeMet in blocking HPV-18 effects.
Methods.
Fertilized mouse embryos were cultured for 5 days to implanted trophoblasts and exposed to either control medium (group 1), HPV-18 (group 2), combined HPV-18 and 0.5 µM SeMet (group 3), or combined HPV-18 and 5.0 µM SeMet (group 4).
After 48 hrs, trophoblast integrity and, apoptosis/necrosis were assessed using morphometric and dual-stain fluorescence assays, respectively.
Results.
HPV-18 exposed trophoblasts nuclei (253.8 ± 28.5 sq·µ) were 29% smaller than controls (355.6 ± 35.9 sq·µ).
Supplementation with 0.5 and 5.0 µM SeMet prevented nuclear shrinkage after HPV-18 exposure.
HPV-18 infected trophoblasts remained larger with SeMet supplementation.
HPV-18 decreased cell viability by 44% but SeMet supplementation sustained cell viability.
Apoptosis was lower when SeMet was present.
HPV-18 decreased inner cell mass (ICM) viability by over 60%.
Conclusions.
HPV-18 decreased nuclear size and trophoblast viability but these effects were attenuated by the antioxidant SeMet.
SeMet blocked HPV-18 associated apoptosis process in trophoblasts but not ICM cells suggesting involvement of different oxidative stress pathways.
American Psychological Association (APA)
Tolen, Jennifer A.& Duerksen-Hughes, Penelope& Lau, Kathleen& Chan, Philip J.. 2015. Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro. International Journal of Reproductive Medicine،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1066953
Modern Language Association (MLA)
Tolen, Jennifer A.…[et al.]. Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro. International Journal of Reproductive Medicine No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1066953
American Medical Association (AMA)
Tolen, Jennifer A.& Duerksen-Hughes, Penelope& Lau, Kathleen& Chan, Philip J.. Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro. International Journal of Reproductive Medicine. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1066953
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066953