Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors

الملخص EN

Mesenchymal stem cells are immunoregulation cells.

IL-22 plays an important role in the pathogenesis of immune thrombocytopenia.

However, the effects of mesenchymal stem cells on IL-22 production in patients with immune thrombocytopenia remain unclear.

Flow cytometry analyzed immunophenotypes of mesenchymal stem cells; differentiation of mesenchymal stem cells was observed by oil red O and Alizarin red S staining; cell proliferation suppression was measured with MTS; IL-22 levels of cell-free supernatants were determined by ELISA.

Mesenchymal stem cells inhibited the proliferation of activated CD4+T cells; moreover, mesenchymal stem cells immunosuppressed IL-22 by soluble cellular factors but not PGE2.

These results suggest that mesenchymal stem cells may be a therapeutic strategy for patients with immune thrombocytopenia.