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A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method
المؤلف
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-11
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
This paper describes a combined antigen/antibody immunoassay implemented in a 96-well plate using fluorescent spectroscopic method.
First, goat anti-human IgG was used to capture human IgG (model antigen); goat anti-human IgG (Cy3 or FITC) was used to detect the model antigen; a saturating level of model antigen was then added followed by unlabelled goat anti-human IgG (model antibody); finally, Cy3 labelled rabbit anti-goat IgG was used to detect the model antibody.
Two approaches were applied to the concomitant assay to analyze the feasibility.
The first approach applied FITC and Cy3 when both targets were present at the same time, resulting in 50 ng/mL of the antibody detection limit and 10 ng/mL of antigen detection limit in the quantitative measurements of target concentration, taking the consideration of FRET efficiency of 68% between donor and acceptor.
The sequential approach tended to lower the signal/noise (S/N) ratio and the detection of the model antigen (lower than 1 ng/mL) had better sensitivity than the model antibody (lower than 50 ng/mL).
This combined antigen/antibody method might be useful for combined detection of antigens and antibodies.
It will be helpful to screen for both antigen and antibody particularly in the situations of the multiserotype and high-frequency mutant virus infections.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cao, Yuan-Cheng. 2015. A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method. Journal of Spectroscopy،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1070271
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cao, Yuan-Cheng. A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method. Journal of Spectroscopy No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1070271
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cao, Yuan-Cheng. A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method. Journal of Spectroscopy. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1070271
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1070271
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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