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A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method
Author
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This paper describes a combined antigen/antibody immunoassay implemented in a 96-well plate using fluorescent spectroscopic method.
First, goat anti-human IgG was used to capture human IgG (model antigen); goat anti-human IgG (Cy3 or FITC) was used to detect the model antigen; a saturating level of model antigen was then added followed by unlabelled goat anti-human IgG (model antibody); finally, Cy3 labelled rabbit anti-goat IgG was used to detect the model antibody.
Two approaches were applied to the concomitant assay to analyze the feasibility.
The first approach applied FITC and Cy3 when both targets were present at the same time, resulting in 50 ng/mL of the antibody detection limit and 10 ng/mL of antigen detection limit in the quantitative measurements of target concentration, taking the consideration of FRET efficiency of 68% between donor and acceptor.
The sequential approach tended to lower the signal/noise (S/N) ratio and the detection of the model antigen (lower than 1 ng/mL) had better sensitivity than the model antibody (lower than 50 ng/mL).
This combined antigen/antibody method might be useful for combined detection of antigens and antibodies.
It will be helpful to screen for both antigen and antibody particularly in the situations of the multiserotype and high-frequency mutant virus infections.
American Psychological Association (APA)
Cao, Yuan-Cheng. 2015. A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method. Journal of Spectroscopy،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1070271
Modern Language Association (MLA)
Cao, Yuan-Cheng. A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method. Journal of Spectroscopy No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1070271
American Medical Association (AMA)
Cao, Yuan-Cheng. A Model System for Concurrent Detection of Antigen and Antibody Based on Immunological Fluorescent Method. Journal of Spectroscopy. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1070271
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1070271