Hydrogen-Rich Saline Protects against IschemiaReperfusion Injury in Grafts after Pancreas Transplantations by Reducing Oxidative Stress in Rats

الملخص EN

Purpose.

This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats.

Methods.

Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats.

The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group).

Samples from the pancreas and blood were taken at 12 hours after reperfusion.

The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines.

Results.

Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats.

Histopathological improvements and recovery of impaired pancreatic function were observed.

In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group.

Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.

Conclusion.

Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.