Hydrogen-Rich Saline Protects against IschemiaReperfusion Injury in Grafts after Pancreas Transplantations by Reducing Oxidative Stress in Rats
Joint Authors
Ren, Jian-Dong
Luo, Zhu-Lin
Cheng, Long
Fang, Chen
Xiang, Ke
Xu, Hao-Tong
Tang, Li-Jun
Wang, Tao
Tian, Fu-Zhou
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Purpose.
This study aimed to investigate the therapeutic potential of hydrogen-rich saline on pancreatic ischemia/reperfusion (I/R) injury in rats.
Methods.
Eighty heterotopic pancreas transplantations (HPT) were performed in syngenic rats.
The receptors were randomized blindly into the following three groups: the HPT group and two groups that underwent transplantation and administration of hydrogen-rich saline (HS, >0.6 mM, 6 mL/kg) or normal saline (NS, 6 mL/kg) via the tail vein at the beginning of reperfusion (HPT + HS group, HPT + NS group).
Samples from the pancreas and blood were taken at 12 hours after reperfusion.
The protective effects of hydrogen-rich saline against I/R injury were evaluated by determining the changes in histopathology and measuring serological parameters, oxidative stress-associated molecules, and proinflammatory cytokines.
Results.
Administration of hydrogen-rich saline produced notable protection against pancreatic I/R injury in rats.
Histopathological improvements and recovery of impaired pancreatic function were observed.
In addition, TNF-α, IL-1β, and IL-6 were reduced markedly in the HPT + HS group.
Additionally, there were noticeable inhibitory effects on the pancreatic malondialdehyde level and considerable recruitment of SOD and GPx, which are antioxidants.
Conclusion.
Hydrogen-rich saline treatment significantly attenuated the severity of pancreatic I/R injury in rats, possibly by reducing oxidative stress and inflammation.
American Psychological Association (APA)
Luo, Zhu-Lin& Cheng, Long& Ren, Jian-Dong& Fang, Chen& Xiang, Ke& Xu, Hao-Tong…[et al.]. 2015. Hydrogen-Rich Saline Protects against IschemiaReperfusion Injury in Grafts after Pancreas Transplantations by Reducing Oxidative Stress in Rats. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1072230
Modern Language Association (MLA)
Luo, Zhu-Lin…[et al.]. Hydrogen-Rich Saline Protects against IschemiaReperfusion Injury in Grafts after Pancreas Transplantations by Reducing Oxidative Stress in Rats. Mediators of Inflammation No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1072230
American Medical Association (AMA)
Luo, Zhu-Lin& Cheng, Long& Ren, Jian-Dong& Fang, Chen& Xiang, Ke& Xu, Hao-Tong…[et al.]. Hydrogen-Rich Saline Protects against IschemiaReperfusion Injury in Grafts after Pancreas Transplantations by Reducing Oxidative Stress in Rats. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1072230
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072230