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Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model
المؤلفون المشاركون
Hamaguchi, Shigeto
Akeda, Yukihiro
Yamamoto, Norihisa
Seki, Masafumi
Yamamoto, Kouji
Oishi, Kazunori
Tomono, Kazunori
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-07-26
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Recently, it has been reported that circulating free DNA (cf-DNA) in the blood is increased in various infectious diseases, including sepsis.
Moreover, a relationship between cf-DNA and neutrophil extracellular traps (NETs) has been suggested.
However, it is still unclear what the source and physiological role of cf-DNA in sepsis are.
In this study, we examined the source of cf-DNA by detecting citrullinated histone H3, a characteristic feature of NET formation, in cecal ligation and puncture- (CLP-)operated mice.
In addition, neutrophil depletion using anti-Ly6G antibodies was performed to assess the association between neutrophils and cf-DNA.
Increased cf-DNA levels were observed only in CLP mice and not in the control groups; the qPCR findings revealed that the cf-DNA was mainly host-derived, even in bacteremic conditions.
Citrullinated histone H3 was not increased in the neutrophils upon CLP, and the depletion of neutrophils showed limited effects on decreasing the amount of cf-DNA.
Taken together, these results suggested that elevated cf-DNA levels during early-phase sepsis may represent a candidate biomarker for the severity of sepsis and that, contrary to previous findings, cf-DNA is not derived from neutrophils or NETs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hamaguchi, Shigeto& Akeda, Yukihiro& Yamamoto, Norihisa& Seki, Masafumi& Yamamoto, Kouji& Oishi, Kazunori…[et al.]. 2015. Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072456
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hamaguchi, Shigeto…[et al.]. Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model. Mediators of Inflammation No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1072456
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hamaguchi, Shigeto& Akeda, Yukihiro& Yamamoto, Norihisa& Seki, Masafumi& Yamamoto, Kouji& Oishi, Kazunori…[et al.]. Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072456
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1072456
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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