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Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model
Joint Authors
Hamaguchi, Shigeto
Akeda, Yukihiro
Yamamoto, Norihisa
Seki, Masafumi
Yamamoto, Kouji
Oishi, Kazunori
Tomono, Kazunori
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Recently, it has been reported that circulating free DNA (cf-DNA) in the blood is increased in various infectious diseases, including sepsis.
Moreover, a relationship between cf-DNA and neutrophil extracellular traps (NETs) has been suggested.
However, it is still unclear what the source and physiological role of cf-DNA in sepsis are.
In this study, we examined the source of cf-DNA by detecting citrullinated histone H3, a characteristic feature of NET formation, in cecal ligation and puncture- (CLP-)operated mice.
In addition, neutrophil depletion using anti-Ly6G antibodies was performed to assess the association between neutrophils and cf-DNA.
Increased cf-DNA levels were observed only in CLP mice and not in the control groups; the qPCR findings revealed that the cf-DNA was mainly host-derived, even in bacteremic conditions.
Citrullinated histone H3 was not increased in the neutrophils upon CLP, and the depletion of neutrophils showed limited effects on decreasing the amount of cf-DNA.
Taken together, these results suggested that elevated cf-DNA levels during early-phase sepsis may represent a candidate biomarker for the severity of sepsis and that, contrary to previous findings, cf-DNA is not derived from neutrophils or NETs.
American Psychological Association (APA)
Hamaguchi, Shigeto& Akeda, Yukihiro& Yamamoto, Norihisa& Seki, Masafumi& Yamamoto, Kouji& Oishi, Kazunori…[et al.]. 2015. Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072456
Modern Language Association (MLA)
Hamaguchi, Shigeto…[et al.]. Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model. Mediators of Inflammation No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1072456
American Medical Association (AMA)
Hamaguchi, Shigeto& Akeda, Yukihiro& Yamamoto, Norihisa& Seki, Masafumi& Yamamoto, Kouji& Oishi, Kazunori…[et al.]. Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072456
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072456