miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8
المؤلفون المشاركون
Hong, Zhongsi
Hong, Haiyu
Liu, Jian
Zheng, Xiaobin
Huang, Mingxing
Li, Chunna
Xia, Jinyu
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-07-22
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Aims.
This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a.
Materials and Methods.
miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR).
The luciferase activity assays were used to determine whether miR-106a binds to 3′UTR of IL-8.
miR-106a mimics and inhibitors were transfected into healthy PBMCs.
IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively.
Results.
The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients.
IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels.
The luciferase activity assays indicated that IL-8 is a target of miR-106a.
Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.
Conclusions.
This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hong, Zhongsi& Hong, Haiyu& Liu, Jian& Zheng, Xiaobin& Huang, Mingxing& Li, Chunna…[et al.]. 2015. miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072469
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hong, Zhongsi…[et al.]. miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8. Mediators of Inflammation No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1072469
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hong, Zhongsi& Hong, Haiyu& Liu, Jian& Zheng, Xiaobin& Huang, Mingxing& Li, Chunna…[et al.]. miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072469
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1072469
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر