miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8

Abstract EN

Aims.

This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a.

Materials and Methods.

miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR).

The luciferase activity assays were used to determine whether miR-106a binds to 3′UTR of IL-8.

miR-106a mimics and inhibitors were transfected into healthy PBMCs.

IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively.

Results.

The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients.

IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels.

The luciferase activity assays indicated that IL-8 is a target of miR-106a.

Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.

Conclusions.

This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.