miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8
Joint Authors
Hong, Zhongsi
Hong, Haiyu
Liu, Jian
Zheng, Xiaobin
Huang, Mingxing
Li, Chunna
Xia, Jinyu
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aims.
This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a.
Materials and Methods.
miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR).
The luciferase activity assays were used to determine whether miR-106a binds to 3′UTR of IL-8.
miR-106a mimics and inhibitors were transfected into healthy PBMCs.
IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively.
Results.
The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients.
IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels.
The luciferase activity assays indicated that IL-8 is a target of miR-106a.
Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects.
Conclusions.
This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8.
American Psychological Association (APA)
Hong, Zhongsi& Hong, Haiyu& Liu, Jian& Zheng, Xiaobin& Huang, Mingxing& Li, Chunna…[et al.]. 2015. miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072469
Modern Language Association (MLA)
Hong, Zhongsi…[et al.]. miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8. Mediators of Inflammation No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1072469
American Medical Association (AMA)
Hong, Zhongsi& Hong, Haiyu& Liu, Jian& Zheng, Xiaobin& Huang, Mingxing& Li, Chunna…[et al.]. miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1072469
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072469