HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation
المؤلفون المشاركون
Mastropietro, Giuliana
Tiscornia, Inés
Perelmuter, Karen
Bollati-Fogolín, Mariela
Astrada, Soledad
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-10
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
The NF-κB is a transcription factor which plays a key role in regulating biological processes.
In response to signals, NF-κB activation occurs via phosphorylation of its inhibitor, which dissociates from the NF-κB dimer allowing the translocation to the nucleus, inducing gene expression.
NF-κB activation has direct screening applications for drug discovery for several therapeutic indications.
Thus, pathway-specific reporter cell systems appear as useful tools to screen and unravel the mode of action of probiotics and natural and synthetic compounds.
Here, we describe the generation, characterization, and validation of human epithelial reporter cell lines for functional studies of NF-κB activation by different pro- and anti-inflammatory agents.
Caco-2 and HT-29 cells were transfected with a pNF-κB-hrGFP plasmid which contains the GFP gene under the control of NF-κB binding elements.
Three proinflammatory cytokines (TNF- α , IL-1β, and LPS) were able to activate the reporter systems in a dose-response manner, which corresponds to the activation of the NF-κB signaling pathway.
Finally, the reporter cell lines were validated using lactic acid bacteria and a natural compound.
We have established robust Caco-2-NF-κB-hrGFP and HT-29-NF-κB-hrGFP reporter cell lines which represent a valuable tool for primary screening and identification of bacterial strains and compounds with a potential therapeutic interest.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mastropietro, Giuliana& Tiscornia, Inés& Perelmuter, Karen& Astrada, Soledad& Bollati-Fogolín, Mariela. 2015. HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1072617
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mastropietro, Giuliana…[et al.]. HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation. Mediators of Inflammation No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1072617
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mastropietro, Giuliana& Tiscornia, Inés& Perelmuter, Karen& Astrada, Soledad& Bollati-Fogolín, Mariela. HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1072617
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1072617
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر