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HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation
Joint Authors
Mastropietro, Giuliana
Tiscornia, Inés
Perelmuter, Karen
Bollati-Fogolín, Mariela
Astrada, Soledad
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-10
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The NF-κB is a transcription factor which plays a key role in regulating biological processes.
In response to signals, NF-κB activation occurs via phosphorylation of its inhibitor, which dissociates from the NF-κB dimer allowing the translocation to the nucleus, inducing gene expression.
NF-κB activation has direct screening applications for drug discovery for several therapeutic indications.
Thus, pathway-specific reporter cell systems appear as useful tools to screen and unravel the mode of action of probiotics and natural and synthetic compounds.
Here, we describe the generation, characterization, and validation of human epithelial reporter cell lines for functional studies of NF-κB activation by different pro- and anti-inflammatory agents.
Caco-2 and HT-29 cells were transfected with a pNF-κB-hrGFP plasmid which contains the GFP gene under the control of NF-κB binding elements.
Three proinflammatory cytokines (TNF- α , IL-1β, and LPS) were able to activate the reporter systems in a dose-response manner, which corresponds to the activation of the NF-κB signaling pathway.
Finally, the reporter cell lines were validated using lactic acid bacteria and a natural compound.
We have established robust Caco-2-NF-κB-hrGFP and HT-29-NF-κB-hrGFP reporter cell lines which represent a valuable tool for primary screening and identification of bacterial strains and compounds with a potential therapeutic interest.
American Psychological Association (APA)
Mastropietro, Giuliana& Tiscornia, Inés& Perelmuter, Karen& Astrada, Soledad& Bollati-Fogolín, Mariela. 2015. HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation. Mediators of Inflammation،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1072617
Modern Language Association (MLA)
Mastropietro, Giuliana…[et al.]. HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation. Mediators of Inflammation No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1072617
American Medical Association (AMA)
Mastropietro, Giuliana& Tiscornia, Inés& Perelmuter, Karen& Astrada, Soledad& Bollati-Fogolín, Mariela. HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation. Mediators of Inflammation. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1072617
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1072617