Prophylactic Subacute Administration of Zinc Increases CCL2, CCR2, FGF2, and IGF-1 Expression and Prevents the Long-Term Memory Loss in a Rat Model of Cerebral Hypoxia-Ischemia

الملخص EN

Prophylactic subacute administration of zinc decreases lipoperoxidation and cell death following a transient cerebral hypoxia-ischemia, thus suggesting neuroprotective and preconditioning effects.

Chemokines and growth factors are also involved in the neuroprotective effect in hypoxia-ischemia.

We explored whether zinc prevents the cerebral cortex-hippocampus injury through regulation of CCL2, CCR2, FGF2, and IGF-1 expression following a 10 min of common carotid artery occlusion (CCAO).

Male rats were grouped as follows: (1) Zn96h, rats injected with ZnCl2 (one dose every 24 h during four days); (2) Zn96h + CCAO, rats treated with ZnCl2 before CCAO; (3) CCAO, rats with CCAO only; (4) Sham group, rats with mock CCAO; and (5) untreated rats.

The cerebral cortex-hippocampus was dissected at different times before and after CCAO.

CCL2/CCR2, FGF2, and IGF-1 expression was assessed by RT-PCR and ELISA.

Learning in Morris Water Maze was achieved by daily training during 5 days.

Long-term memory was evaluated on day 7 after learning.

Subacute administration of zinc increased expression of CCL2, CCR2, FGF2, and IGF-1 in the early and late phases of postreperfusion and prevented the CCAO-induced memory loss in the rat.

These results might be explained by the induction of neural plasticity because of the expression of CCL2 and growth factors.